mouse anti pik3r1  (Bioss)

Journal: Poultry Science

Article Title: Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways

doi: 10.1016/j.psj.2024.103696

Figure Lengend Snippet: Information about primary antibodies.

Article Snippet: PI3K , 1:2000 , bsm-33219M , Bioss , China.

Techniques:

Journal: International Journal of Women's Health

Article Title: Molecular Mechanisms Behind Vascular Mimicry as the Target for Improved Breast Cancer Management

doi: 10.2147/IJWH.S406327

Figure Lengend Snippet: Primer Sequences for Real-Time PCR Used in the Study

Article Snippet: After deparaffinization, dehydration, antigen retrieval and blocking endogenous peroxidase, the sections were incubated with primary antibodies against EphA2 (1:500, ZENBIO, 220092), CTNNB1 (1:500, ZENBIO, R22820) and PIK3R1 (1:500, Bioss, Bsm-33219M) at 4°C overnight.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: International Journal of Women's Health

Article Title: Molecular Mechanisms Behind Vascular Mimicry as the Target for Improved Breast Cancer Management

doi: 10.2147/IJWH.S406327

Figure Lengend Snippet: Expression level of the prognostic value in normal tissues and breast cancer samples from the TCGA Database. ( A ) EPHA2, ( B ) PIK3R1, ( C ) CTNNB1, ( D ) PECAM1.

Article Snippet: After deparaffinization, dehydration, antigen retrieval and blocking endogenous peroxidase, the sections were incubated with primary antibodies against EphA2 (1:500, ZENBIO, 220092), CTNNB1 (1:500, ZENBIO, R22820) and PIK3R1 (1:500, Bioss, Bsm-33219M) at 4°C overnight.

Techniques: Expressing

Journal: International Journal of Women's Health

Article Title: Molecular Mechanisms Behind Vascular Mimicry as the Target for Improved Breast Cancer Management

doi: 10.2147/IJWH.S406327

Figure Lengend Snippet: Survival curves comparing low and high expressions of EphA2/PIK3R1/CTNNB1/PECAM1 in breast cancer. ( A ) EPHA2, ( B ) PIK3R1, ( C ) CTNNB1, ( D ) PECAM1. All the survival analyses were conducted via Kaplan–Meier Plotter database ( https://kmplot.com/analysis ).

Article Snippet: After deparaffinization, dehydration, antigen retrieval and blocking endogenous peroxidase, the sections were incubated with primary antibodies against EphA2 (1:500, ZENBIO, 220092), CTNNB1 (1:500, ZENBIO, R22820) and PIK3R1 (1:500, Bioss, Bsm-33219M) at 4°C overnight.

Techniques:

Journal: International Journal of Women's Health

Article Title: Molecular Mechanisms Behind Vascular Mimicry as the Target for Improved Breast Cancer Management

doi: 10.2147/IJWH.S406327

Figure Lengend Snippet: Expression level and correlation analysis of studied genes in breast cancer tissue. ( A – C ) mRNA expression of EphA2/PIK3R1/CTNNB1 by qRT-PCR assay, n=20, *Denotes P <0.05 compared with paracancerous group. ( D – F ) Pearson correlation analysis of EphA2/PIK3R1/CTNNB1/ PECAM1 in breast cancer samples from TCGA database. ( G ) The correlation of EPHA2 and PIK3R1 in breast cancer samples, n=20, R 2 =0.4257, P =0.0018. ( H ) The correlation of PIK3R1and CTNNB1 in breast cancer samples, n=20, R 2 =0.2215, P =0.0362. ( I ) The correlation of EPHA2 and CTNNB1 in breast cancer samples, n=20, R 2 =0.2279, P =0.0333.

Article Snippet: After deparaffinization, dehydration, antigen retrieval and blocking endogenous peroxidase, the sections were incubated with primary antibodies against EphA2 (1:500, ZENBIO, 220092), CTNNB1 (1:500, ZENBIO, R22820) and PIK3R1 (1:500, Bioss, Bsm-33219M) at 4°C overnight.

Techniques: Expressing, Quantitative RT-PCR

Journal: International Journal of Women's Health

Article Title: Molecular Mechanisms Behind Vascular Mimicry as the Target for Improved Breast Cancer Management

doi: 10.2147/IJWH.S406327

Figure Lengend Snippet: Immunohistochemical staining of studied genes in para-carcinoma and breast cancer tissues. ( A ) Expression intensity and location of EphA2 in para-carcinoma tissue and breast cancer tissue by immunohistochemical staining assay. ( B ) Quantitative analysis of EphA2 from immunohistochemical images by histochemical scores. ( C ) Expression intensity and location of PIK3R1 in para-carcinoma tissue and breast cancer tissue by immunohistochemical staining assay. ( D ) Quantitative analysis of PIK3R1 from immunohistochemical images by histochemical scores. ( E ) Expression intensity and location of CTNNB1 in para-carcinoma tissue and breast cancer tissue by Immunohistochemical staining assay. ( F ) Quantitative analysis of CTNNB1 from immunohistochemical images by histochemical scores. n=20, *Denotes P <0.05 compared with paracancerous group.

Article Snippet: After deparaffinization, dehydration, antigen retrieval and blocking endogenous peroxidase, the sections were incubated with primary antibodies against EphA2 (1:500, ZENBIO, 220092), CTNNB1 (1:500, ZENBIO, R22820) and PIK3R1 (1:500, Bioss, Bsm-33219M) at 4°C overnight.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: International Journal of Women's Health

Article Title: Molecular Mechanisms Behind Vascular Mimicry as the Target for Improved Breast Cancer Management

doi: 10.2147/IJWH.S406327

Figure Lengend Snippet: The correlation of vascular mimicry and PIK3R1/CTNNB1 in breast cancer. ( A ) vasculogenic mimicry exists in paracancerous and breast cancer tissues. ( B ) VM in moderate and strong expression of PIK3R1 in cancer tissues. ( C ) Quantitative analysis of VM channel in moderate and strong expression of PIK3R1, **Denotes P <0.01 compared with moderate group. ( D ) VM in moderate and strong expression of CTNNB1 in cancer tissues. ( E ) Quantitative analysis of VM channel in moderate and strong expression of CTNNB1, *Denotes P <0.05 compared with moderate group. ( F and G ) Expression of PIK3R1 and CTNNB1 in different VM channels by qRT-PCR assay. *And **Denote P <0.05 and P <0.01 compared with VM (0–2) group.

Article Snippet: After deparaffinization, dehydration, antigen retrieval and blocking endogenous peroxidase, the sections were incubated with primary antibodies against EphA2 (1:500, ZENBIO, 220092), CTNNB1 (1:500, ZENBIO, R22820) and PIK3R1 (1:500, Bioss, Bsm-33219M) at 4°C overnight.

Techniques: Expressing, Quantitative RT-PCR